Tanta University - Egypt. September 2010.
- The main active ingredient of black cumin is thymoquinone which is an antioxidant.
- The experiment was performed with 76 male Wistar rats for a period of 8.5 months.
- Five different genotoxic carcinogens were given to subjects in order to run a multi-organ experiment and induce tumors:
- DEN (Diethylnitrosamine) : Liver
- MNU (N-methyl-N-nitrosourea) : Lung + Gastrointestinal
- DHPN (2,2'-dihydroxy-di-N-propylnitrosamine) : Lung
- DMH (1,2-dimethylhydrazine) : Colon
- BBN (N-butyl-N-(3-hydroxybutyl)nitrosamine) : Bladder
- At the end of the experiment subjects' organs were excised and tumors were measured.
- 76 male Wistar rats were divided into 5 groups according to their body weight. (20 rats each for groups 1-3, 8 rats each for groups 4-5)
- Groups 1-3 were exposed to various carcinogens for 4 weeks and then given either no treatment or doses of 4,000 ppm or 1,000 ppm of black cumin essential oil extract in their diet.
- Groups 4-5 were not given carcinogens but were given injections of saline instead and used as control groups.
- No tumors were found in control groups 4 & 5.
- No unfavorable side effects seen in the use of black cumin oil in the 8+ month study.
- Daily doses of black cumin oil mixed into the diet of Wistar rats significantly inhibited tumor development in multiple sites of the rat body:
- Colon: 46.6% - 49% less tumors in Group 1 & 2 vs Group 3.
- Lung: 50% - 52.7% less tumors in Group 1 & 2 vs Group 3.